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(D) Mean heart size (wet weight) of 12-month-old mice raised on LCD or HCD (n = 10–13 per group).

(E) Mean fasted serum leptin concentrations of 12-month-old mice raised on HCD (n = 10).

(B) H&E staining of representative left liver lobe sections of 12-month-old mice raised on LCD (scale bar: 200 μM).

Although guanylyl cyclase and downstream c GMP are essential regulators of centrally regulated feeding behavior in invertebrates, the role of this primordial signaling mechanism in mammalian appetite regulation has eluded definition.(F) Correlation of fasted serum leptin level and body weight of 12-month-old mice raised on HCD (r mice were hyperphagic, independent of sex, dietary nutrients, or dietary caloric content (Figure 3A).